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For Laboratory Researchers – Laboratory References


Boyle-Vavra S, Yin S, Jo DS, Montgomery C, Daum RS. VraT/YvqF is required for methicillin resistance and activation of the VraSR regulon in Staphylococcus aureus. Antimicrob Agents Chemother. 2012 Oct 15. [Epub ahead of print].

Molinero LL, Cubre A, Mora C, Alegre ML. TCR/CARMA1/NF- B signaling controls Th17 differentiation. Proc. Natl. Acad. Sci. U.S.A., 2012, in press.

Molinero LL, Alegre ML.  Role of T cell-nuclear factor kB in transplantation.  Transplant. Reviews 26:189-200, 2012.

Alegre ML, Molinero LL.  The inside story of dendritic cell immunotherapy.  Editorial in Am. J. Transplant., 12:1363-1364, 2012.

Montgomery CP, Boyle-Vavra S, Roux A, Ebine K, Sonenshein AL, Daum RS. CodY Deletion Enhances In Vivo Virulence of Community-Associated Methicillin-Resistant Staphylococcus aureus Clone USA300. Infection and Immunity 2012 Jul;80(7):2382-2389.

Lozano Porras D, Wang Y, Molinero LL*, Alegre ML*. Role of T cell-specific NF-kB in islet allograft rejection. Transplantation, 93:976-82, 2012. (*co-last authors).

Alegre ML, Bromberg J. The anti-infectious role of the IL-17 pathway. Literature Watch. American Journal of Transplantation, 12:3, 2012.

Alegre ML, Bromberg J. Commensal microbiota determine intestinal iTreg. Literature Watch. American Journal of Transplantation, in press, 2012.

Alegre, ML, Chong AS. Costimulatory molecules and Toll-like receptors in transplantation. American Society of Transplantation Handbook of Transplant Immunology, Wiley-Blackwell, 2012, in press.

Alegre, ML. Mechanisms of allograft destruction: cellular mechanisms of adaptive immunity. Textbook on Organ Transplantation, Wiley-Blackwell, 2012, in press.

Alegre ML, Molinero LL. The inside story of dendritic cell immunotherapy. Editorial in Am. J. Transplantation, 12:1363-1364, 2012.

Chong AS, Alegre ML. The impact of infection and tissue damage in solid organ transplantation. Nature Reviews in Immunology, 12:459-71, 2012.


Ahmed EB, Wang T, Tao J, Alegre ML, Chong AS. IL-6 induced by Staphylococcus aureus infection prevents the induction of skin allograft acceptance in mice. Am. J. Transplant., 11:936-946, 2011.

Molinero LL, Miller ML, Evaristo C, Alegre ML. High TCR Stimuli Prevent iTreg Differentiation in an NF-kB-Dependent Manner. J. Immunol., 186: 4609-4617, 2011.

Ahmed EB, Daniels, M, Alegre ML, Chong AS. Bacterial infections, alloimmunity and transplantation tolerance. Transplantation Reviews, 25:27-35, 2011.

Molinero LL, Alegre ML. NF-kB in transplantation. Transplantation Reviews, 2011, in press.

Alegre ML, Fu YX. Attenuation by targeting the B and T cell attenuator (BTLA). Commentary in Transplantation, 92:1075-62011, 2011.

Alegre ML, Chong AS. “Tip-toeing” to an assay for transplantation tolerance? Editorial in American Journal of Transplantation, 12:519-20, 2011.

Jo DS, Montgomery CP, Yin S, Boyle-Vavra S, Daum RS. Improved oxacillin treatment outcomes in experimental skin and lung infection by a methicillin resistantStaphylococcus ureus isolate with a vraSR operon deletion. Antimicrob Agents Chemother 2011 Jun;55(6):2818-23.

Boyle-Vavra S, Jones M, Gourley BL, Holmes M, Ruf R, Balsam AR, Boulware DR, Kline S, Jawahir S, Devries A, Peterson SN, Daum RS. Comparative genome sequencing of an isogenic pair of USA800 clinical methicillin-resistant Staphylococcus aureus isolates obtained before and after daptomycin treatment failure. Antimicrob Agents Chemother 2011 May;55(5):2018-25.

Cadilla A, David MZ, Daum RS, Boyle-Vavra S. Association of high-level
mupirocin resistance and multidrug-resistant methicillin-resistant Staphylococcus aureus at an academic center in the midwestern United States. J Clin Microbiol 2011 Jan;49(1):95-100.

Alegre ML, Fu YX. Attenuation by targeting the B and T cell attenuator (BTLA). Commentary in Transplantation, 2011, in press.

Molinero LL, Alegre ML. NF-kB in transplantation. Transplantation Reviews, 2011, in press.

Ahmed EB, Wang T, Tao J, Alegre ML, Chong AS. IL-6 induced by Staphylococcus aureus infection prevents the induction of skin allograft acceptance in mice.  Am. J. Transplant. 2011;11:936-946.

Molinero LL, Miller ML, Evaristo C, Alegre ML. High TCR Stimuli Prevent iTreg Differentiation in an NF-kB-Dependent Manner. J. Immunol., 2011;186:4609-4617.  Featured in the “In This Issue” article highlights.

Ahmed EB, Daniels, M, Alegre ML, Chong AS. Bacterial infections, alloimmunity and transplantation tolerance. Transplantation Reviews 2011;25:27-35.


Boyle-Vavra S, Daum RS. Reliability of the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) assay in detecting MRSA isolates with a variety of genotypes from the United States and Taiwan. J Clin Microbiol 2010 Dec;48(12):4546-51.

Yang SJ, Xiong YQ, Boyle-Vavra S, Daum R, Jones T, Bayer AS.
Daptomycin-oxacillin combinations in treatment of experimental endocarditis caused by daptomycin-nonsusceptible strains of methicillin-resistant Staphylococcus aureus with evolving oxacillin susceptibility (the “seesaw effect”). Antimicrob Agents Chemother 2010 Aug;54(8):3161-9.

Bhorade S, Chen H, Molinero LL, Liao C, Garrity ER, Vigneswaran WT, Shilling R, Sperling A, Chong AS, Alegre ML.  Decreased percentage of CD4+FoxP3+ cells in BAL from lung transplant recipients correlates with development of BOS.  Transplantation 2010;90:540-546.

Wang T, Ahmed E, Chen L, Wang CR, Alegre ML, Chong AS.  Infection with the intracellular bacterium, Listeria monocytogenes overrides established allograft tolerance.  Am. J. Transplant. 2010;10:1524-1533.  Accompanying editorial by Ron Gill AJT, 10:1499-1500 “Just How Stable Is Allograft Tolerance?”

Montgomery CP, Boyle-Vavra S, Daum RS.  Importance of the global regulators Agr and SaeRS in the pathogenesis of CA-MRSA USA300 infection.  PLoS ONE. 2010 Dec 2;5(12):e15177.


Montgomery CP, Boyle-Vavra S, Daum RS.  The arginine catabolic mobile element (ACME) is not associated with enhanced virulence in experimental invasive disease caused by the community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) genetic background USA300.  Infect Immun 2009;77:2650-2656.

Montgomery CP, Daum RS.  Transcription of inflammatory genes in the lung after infection with community-associated methicillin-resistant Staphylococcus aureus: A role for Panton-Valentine Leukocidin?  Infect Immun 2009;77:2159-2167.


Montgomery CP, Boyle-Vavra S, Adem PV, Lee JC, Husain AN, Clasen J, Daum RS.  Comparison of virulence between community-associated methicillin-resistant Staphylococcus aureus pulsotypes USA300 and USA400 in a rat model of pneumonia.  J Infect Dis 2008;198:561-570.

David MZ, Hennessey T, Rudolph K, King K, Boyle-Vavra.  Molecular epidemiology of methicillin-resistant Staphylococcus aureus, rural southwestern Alaska.  Emerg Infect Dis 2008;14:1693-1699.

Daum RS.  Removing the Golden Coat of Staphylococcus aureus.  N Eng J Med (Clinical Implications of Basic Research) 2008;359:85-87.


Boyle-Vavra S, Daum RS.  Community-acquired methicillin-resistant Staphylococcus aureus and the role of Panton-Valentine leukocidin in disease. "Pathobiology in Focus," Lab Invest, 2007;87:3-9.


Yin S, Daum RS, Chaurushiya M, Boyle S.  Characterization of the autoinducible VraSR two-component regulatory system and its role in transcriptional induction of pbb2 by ß-lactams and vancomycin using lacZ fusions on solid and liquid medium in Staphylococcus aureus Antimicrob Ag Chemother 2006;50:336-343.

Boyle-Vavra S, Yin S, Daum RS.  The VraS/VraR two-component regulatory system required for oxacillin resistance in community-acquired methicillin-resistant Staphylococcus aureus.  FEMS Microbiol Lett 2006;262:163-171.


Boyle S, Ereshefsky B, Wang CC, Daum RS.  A successful multi-resistant community associated MRSA lineage from Taipei, Taiwan that carries either a novel staphylococcal chromosomal cassette SCCmec type VT or SCCmec type IV.  J Clin Micro 2005;42:4719-4730.


Mongkolrattonathai K, Boyle S, Murphy TV, Daum RS.  Novel non mecA-containing SCC-composite island containing pbp4 and tagF genes in a commensal staphylococcal species: a possible reservoir for antibiotic resistance islands in Staphylococcus aureus.  Antimicrob Ag Chemother 2004;48:1823-1836.


Boyle-Vavra S, Yin S, Challapalli M, Daum RS.  Transcriptional induction of the penicillin-binding protein 2 gene in Staphylococcus aureus by cell wall active antibiotics oxacillin and vancomycin.  Antimicrob Ag Chemother 2003;47:1028-1036.

Boyle-Vavra S, Challapalli M, Daum RS.  Resistance to autolysis in vancomycin-selected Staphylococcus aureus isolates precedes vancomycin-intermediate resistance.  Antimicrob Ag Chemother 2003;47:2036-2039.

Mongkolrattonathai K, Boyle S, Kahana MD, Daum RS.  Severe Staphylococcus aureus infection caused by clonally-related community-acquired methicillin-susceptible and methicillin resistant isolates. Clin Inf Dis 2003;22:1050-1058.


Ma XX, Ito T, Tiensasitorn C, Jamklang M, Chongtrakool  P, Boyle-Vavra S, Daum RS, Hiramatsu K.  A novel type of staphylococcal cassette chromosome mec (SCCmec) identified in community-acquired methicillin-resistant Staphylococcus aureus strains. Antimicrob Ag Chemother 2002;46:1147-1152.

Hussain FM, Boyle-Vavra S, Shete PB, Daum RS.  Evidence for a continuum of decreased vancomycin susceptibility in unselected Staphylococcus aureus clinical isolates.  J Infect Dis 2002;186:661-667.

Daum RS, Ito T, Hiramatsu K, Mongkolrattonathai K, Hussain F, Boyle-Vavra S.  A novel staphylococcal chromosomal cassette containing mec is present in community acquired methicillin-resistant Staphylococcus aureus isolates in Chicago.  J Infect Dis 2002;186:1344-1377.


Boyle-Vavra  S, Labischinski H, Ebert  CC, Ehlert K, Daum RS.  A spectrum of changes occurs in peptidoglycan composition of glycopeptide-intermediate-resistant clinical Staphylococcus aureus isolates.  Antimicrob Ag Chemother 2001;45:280-287.

Boyle-Vavra S, Carey R, Daum RS.  Development of vancomycin and lysostaphin resistance in a methicillin-resistant Staphylococcus aureus isolate.  J Antimicrob Chemother 2001;48:617-625.


Boyle-Vavra S, Berke SK, Daum RS.  Reversion of glycopeptide resistance phenotype in Staphylococcus aureus clinical isolates.  Antimicrob Ag Chemother 2000;44:272-277.

Boyle-Vavra S, Hahm J, Sibener SJ, Daum RS.  Structural and topological differences between a glycopeptide-intermediate clinical strain and glycopeptide-susceptible strains of Staphylococcus aureus revealed by atomic force microscopy. Antimicrob Ag Chemother 2000;44:3456-3460.


Hanaki H, Kuwahara-Arai K, Boyle-Vavra S, Daum RS, Tenover FC, Labischinski H, Hiramatsu K.  Activated cell-wall synthesis is associated with vancomycin resistance in methicillin-resistant Staphylococcus aureus clinical strains Mu3 and Mu50.  J Antimicrob Chemother 1998;42:199-209.


Moreira B, Boyle-Vavra S, deJonge BLM, Daum RS.  Increased production of penicillin-binding protein 2, increased detection of other penicillin-binding proteins, and decreased coagulase activity associated with glycopeptide resistance in Staphylococcus aureus.  Antimicrob Ag Chemother 1997;41:1788-1793.

Boyle-Vavra S, de Jonge BLM, Ebert C, Daum RS.  Cloning of the Staphylococcus aureus ddh gene encoding NAD+-dependent D-lactate dehydrogenase and insertional inactivation in a glycopeptide resistant S aureus isolate.  J Bacteriol 1997;179:6756-6763.


Milewski WM, Boyle-Vavra S, Moreira B, Ebert C, Daum RS.  Overproduction of a 37kDa cytoplasmic protein homologous to NAD+ -linked D-lactate dehydrogenase associated with vancomycin resistance in Staphylococcus aureus.  Antimicrobial Agents and Chemotherapy 1996;40:166-172.


Daum RS, Fattom A, Freese S, Karakawa W.  Capsular polysaccharide serotypes of coagulase positive staphylococci associated with tenosynovitis, osteomyelitis and other invasive infections in chickens and turkeys: evidence for new capsular types.  Avian Diseases 1994;38:762-771.


Daum RS, Gupta S, Sabbagh R, Milewski WM.  Characterization of Staphylococcus aureus isolates with decreased susceptibility to vancomycin and teicoplanin: isolation and purification of a constitutively produced protein associated with decreased susceptibility.  J Infect Dis 1992;166:1066-1072.


Daum RS, Davis H, Shane S, Mulvihill D, Campeau R, Farris B.  Bacteremia and osteomyelitis in an avian model of S. aureus infection.  J Orthop Res 1990;8:804-813.